Spot Indole Test: Result, Principle, Procedure, and Reagents
Aug 18, 2021 · Spot Indole Test. Spot Indole test is used to determine the presence of an enzyme tryptophanase, which helps in breaking the tryptophan to release indole which on reacting with cinnamaldehyde forms a blue-green compound. However, the absence of an enzyme results in no change in color, which represents the indole negative.
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